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. 2011 Sep 13;286(44):38159–38167. doi: 10.1074/jbc.M111.281006

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — N-terminal cytoplasmic region of ATP9B is required for its Golgi localization. HeLa cells were transfected with an expression vector for HA-tagged ATP9A, ATP9B, or a chimeric construct of these proteins (ATP9AB or ATP9BA) schematically shown on the right side. Samples were doubly stained for HA and TGN46 and processed for immunofluorescence microscopy (A). Bar, 20 μm. Cells transfected and immunostained as in A were scored for distribution patterns of ER, ER + Golgi, Golgi, Golgi + puncta, and puncta (B). In each transfection, 200–350 cells were counted. The values are percentages of cells showing the specified distribution patterns of ATP9 constructs.