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Three-dimensional reconstruction of TRPA1. The final 3D reconstruction of TRPA1 is presented at a threshold corresponding to a molecular mass of 525 kDa. The proposed transmembrane domain (TM, blue) and cytoplasmic domain (CP, pink) are indicated. Side, cut-away, top, and bottom views of the reconstruction are shown. The cut-away view shows the internal cavity in the TRPA1 EM density. Segmentation was accomplished using Segger. Scale bar = 10 nm.
