TABLE 2.
Peptidase activities of recombinant PeDPP11 and fraction 52 from DEAE-Sephacel chromatography
Neuromedin B and its derivatives were separately incubated with recombinant PeDPP11 or fraction 52 for 1 h at 30 °C. Amounts of the peptide products were semiquantitatively analyzed by MALDI-TOF MS. Substituted amino acid residues from neuromedin B are underlined.
| Substrate | Sequence | Specimen | Degradationa |
|---|---|---|---|
| % | |||
| Neuromedin B | GNLWATGHFM-NH2 | PeDPP11 | 0 |
| Asp1-neuromedin B | DGLWATGHFM-NH2 | PeDPP11 | 0 |
| Asp2-neuromedin B | GDLWATGHFM-NH2 | PeDPP11 | 100 |
| Asp3-neuromedin B | GSDWATGHFM-NH2 | PeDPP11 | 0 |
| Asp5-neuromedin B | GSLWDTGHFM-NH2 | PeDPP11 | 0 |
| Neuromedin B | GNLWATGHFM-NH2 | Fraction 52b | 5 |
| Asp2-neuromedin B | GDLWATGHFM-NH2 | Fraction 52b | 100 |
a Expressed as percent of fragment products losing N-terminal moiety.
b Fraction 52 was from DEAE-Sephacel chromatography (Fig. 3A).