TABLE 2.
Arterial levels of lactate, alanine, serine, threonine, glycine, glycerol, and free fatty acids (FFAs) before (control) and after drug treatment (Test Period I) and subsequent increase of plasma glucagon (Test Period II) in 18-h fasted conscious dogs
Blood (BL) and plasma concentration (PL) of metabolites are described in micromolars. Values are means ± S.E. for five experiments. V, vehicle.
| Metabolites | Group | Control | Study Period |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Test Period I |
Test Period II |
||||||||||
| 30 | 60 | 90 | 100 | 120 | 150 | 180 | 210 | ||||
| Lactate | V | BL | 495 ± 30 | 504 ± 64 | 448 ± 26 | 465 ± 61 | 632 ± 49 | 577 ± 53 | 506 ± 51 | 511 ± 39 | 609 ± 87 |
| GPI | BL | 810 ± 150 | 608 ± 93 | 578 ± 112 | 471 ± 129 | 567 ± 111 | 662 ± 174 | 587 ± 156 | 523 ± 100 | 508 ± 117 | |
| MT | BL | 830 ± 111 | 822 ± 125 | 849 ± 115 | 903 ± 121 | 981 ± 159 | 1042 ± 159 | 952 ± 125 | 1030 ± 224 | 986 ± 187 | |
| Alanine | V | BL | 507 ± 47 | 553 ± 28 | 503 ± 39 | 521 ± 58 | 556 ± 32 | 543 ± 39 | 487 ± 32 | 426 ± 61 | 394 ± 43 |
| GPI | BL | 509 ± 74 | 457 ± 73 | 398 ± 71 | 388 ± 65 | 408 ± 92 | 428 ± 123 | 369 ± 104 | 308 ± 84 | 311 ± 87 | |
| MT | BL | 556 ± 26 | 577 ± 36 | 540 ± 23 | 544 ± 38 | 582 ± 83 | 611 ± 53 | 567 ± 64 | 563 ± 42 | 501 ± 66 | |
| Serine | V | BL | 198 ± 19 | 187 ± 10 | 194 ± 19 | 180 ± 15 | 165 ± 12 | 154 ± 14 | 152 ± 12 | 142 ± 8 | 127 ± 8 |
| GPI | BL | 174 ± 14 | 174 ± 22 | 184 ± 29 | 177 ± 14 | 148 ± 20 | 133 ± 13 | 144 ± 20 | 128 ± 14 | 132 ± 14 | |
| MT | BL | 188 ± 26 | 174 ± 26 | 176 ± 27 | 181 ± 25 | 135 ± 35 | 165 ± 17 | 155 ± 19 | 153 ± 18 | 136 ± 21 | |
| Threonine | V | BL | 276 ± 32 | 264 ± 36 | 287 ± 39 | 269 ± 31 | 242 ± 22 | 228 ± 15 | 234 ± 23 | 212 ± 24 | 190 ± 14 |
| GPI | BL | 277 ± 36 | 270 ± 51 | 288 ± 58 | 262 ± 31 | 248 ± 45 | 216 ± 32 | 224 ± 42 | 203 ± 32 | 213 ± 42 | |
| MT | BL | 303 ± 47 | 287 ± 36 | 296 ± 29 | 299 ± 38 | 197 ± 53 | 298 ± 33 | 255 ± 14 | 269 ± 23 | 242 ± 34 | |
| Glycine | V | BL | 267 ± 27 | 253 ± 21 | 263 ± 26 | 250 ± 19 | 233 ± 19 | 214 ± 19 | 207 ± 20 | 193 ± 16 | 171 ± 15 |
| GPI | BL | 253 ± 32 | 257 ± 39 | 264 ± 48 | 269 ± 39 | 221 ± 33 | 189 ± 22 | 210 ± 42 | 176 ± 26 | 183 ± 27 | |
| MT | BL | 285 ± 30 | 278 ± 30 | 281 ± 24 | 278 ± 24 | 212 ± 56 | 262 ± 12 | 229 ± 15 | 230 ± 18 | 195 ± 22 | |
| Glycerol | V | PL | 124 ± 14 | 112 ± 12 | 104 ± 10 | 110 ± 15 | 127 ± 15 | 98 ± 5 | 73 ± 10 | 93 ± 15 | 125 ± 26 |
| GPI | PL | 164 ± 14 | 195 ± 24 | 155 ± 13 | 180 ± 10 | 180 ± 12 | 144 ± 23 | 158 ± 19 | 149 ± 15 | 157 ± 23 | |
| MT | PL | 171 ± 29 | 152 ± 19 | 156 ± 25 | 149 ± 18 | 158 ± 18 | 156 ± 17 | 131 ± 16 | 139 ± 15 | 132 ± 14 | |
| FFAs | V | PL | 285 ± 75 | 244 ± 62 | 297 ± 51 | 225 ± 51 | 222 ± 56 | 221 ± 61 | 164 ± 47 | 158 ± 33 | 271 ± 93 |
| GPI | PL | 315 ± 82 | 304 ± 73 | 311 ± 61 | 383 ± 72 | 355 ± 58 | 283 ± 69 | 229 ± 48 | 211 ± 76 | 204 ± 87 | |
| MT | PL | 331 ± 85 | 254 ± 53 | 258 ± 68 | 239 ± 35 | 224 ± 59 | 203 ± 58 | 144 ± 17 | 153 ± 29 | 211 ± 47 | |