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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Chem Educ. 2011 Jan 1;88(1):95–97. doi: 10.1021/ed900054b

Figure 2.

Figure 2

300 MHz proton spectrum of α1-acid glycoprotein in 90% D2O. At 3.4–4.2 ppm, there is a large contaminating buffer peak in addition to carbohydrate resonances. The envelope of the protein amino acid resonances can be seen. The large singlet at ~ 2ppm is from the methyl protons of N-acetyl groups on some of the sugars (N-acetylglucosamine, N-acetylneuraminic acid).