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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Chem Educ. 2011 Jan 1;88(1):95–97. doi: 10.1021/ed900054b

Figure 5.

Figure 5

Intensities of the N-acetate signals corresponding to the fetuin-conjugated N-acetylneuraminic acid (Substrate) and the free N-acetylneuraminic acid (Product). The intensities are measured from a single frequency in the 1D NMR spectra and include background glycoprotein signals that do not change over time. The change of these intensities over time is the salient feature of this experiment. An exponential decay described by the equation I(x) = −(Ie − Is) e−dx + Ie was fitted to these curves to determine a half-life for reaction, where “I” is the intensity of the signal at time “x,” “Is” is the intensity before the reaction, “Ie” is the intensity at the end of the reaction, and “d” is the decay constant.