Hsp90, Hop, and FKBP52 interact to form binary and ternary complexes. MS recorded after t = 0 to t = 45 min showing the formation of the asymmetric complex containing both Hop and FKBP52. (Hsp90)2(Hop)1 and (Hsp90)2(Hop)2 were formed with twofold excess of Hop prior to the addition of FKBP52. Charge states colored as follows: Hop (light green), Hsp90 monomer and dimer (gray and black respectively), (Hsp90)2(Hop)1 dark green, (Hsp90)2(Hop)2 purple, (Hsp90)1(FKBP52)1 yellow, (Hsp90)2(FKBP2)1 blue, and (Hsp90)2(Hop)1(FKBP52)1 red. Same colors used throughout.