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Phosphorylation of ACC, AMPK, and Kv2.1 in response to A-769662 in isogenic HEK293 cells expressing Kv2.1 (duplicate cell incubations). (A) Effect of A-769662 concentration. (B) Time course. (C) Phosphorylation of S440/S537 in cells expressing either WT Kv2.1 or an S440A/S537A double mutant. Panels labeled pACC, pAMPK, pS440, and pS537 are Western blots obtained using appropriate phosphospecific antibodies.
