Fig. 4.

Voltage–conductance plots showing effects of AMPK on voltage dependence of activation/inactivation in cells expressing WT or S440A/S537A single/double substitutions. (A–D) Effects of preincubation in the bath with A-769662 (100 μM, 20 min) ± compound C (40 μM). (E and F) Effect of intracellular dialysis with thiophosphorylated active or inactive AMPK introduced via the patch pipette. Individual data points are mean ± SEM (n = 7–10); results were fitted to the sigmoidal Boltzmann equation, and the curves were generated using the parameters shown in Table S2.