Fig. 1.

Naturally intronless HSPB3 mRNA accumulates in the cytoplasm in a sequence-dependent manner. (A) Schematic of CMV HSPB3 and its size-matched counterpart CMV Random 1. Vector sequences are shown as gray lines. BGH pA, bovine growth hormone polyA site. (B) FISH was used to determine the nucleocytoplasmic distribution of HSPB3 mRNA after transiently transfecting HeLa cells for the times indicated. DAPI staining was used to identify the nucleus, and the merged images are shown in Bottom. (Scale bar: 10 μm.) (C) FISH comparison of HSPB3 and Random 1 transcripts at 9 or 24 h after transient transfection at lower magnification. (Scale bar: 10 μm.)