Fig. 5.

Multiple paths to GK functional evolution. (A) Fluorescence quenching upon GMP binding to the GK enzyme. Data are displayed as in Fig. 4A. See Fig. S2 for full emission spectra. In the S33P mutation, quenching by GMP is not observed even at high concentrations. (B) Mutation of Dm S66 to proline causes gain of spindle-orienting function. Cumulative percentage graph and symbols are shown as in Fig. 1F. Asterisks denote statistical significance (** represents P < 0.01, * represents P < 0.05; Dunnett’s post hoc test) from +dlg RNAi.