Fig. 2.

Fates of sister chromatids of a single chromosome during anaphase. HCT116 cells in anaphase expressing lacIGFP with LacO arrays integrated into a single chromosome were fixed and imaged after inducing high levels of merotely with MCAK siRNA or monastrol washout. Representative images show the marked chromosome segregating correctly, with one chromosome in each anaphase plate (A), lagging in the spindle midzone (B), or missegregating with no evidence of lagging (C). Arrowheads point to the LacIGFP labeled chromosome. (Scale bar: 5 μm.)