Table 1.

Kinetic constant of Rab-LidA interactions

Rab	kon, 106 M-1 s-1	koff, s-1	Kd, nM	Signal
Rab1∶GDP	—	—	< 10*	ITC
Rab1-AMP∶GDP	—	—	< 10*	ITC
Rab1∶mGDP	20.2	< 10-4*	< 0.01*	Fl
Rab1-AMP∶mGDP	8.2	< 10-4*	< 0.01*	FP
Rab1∶mGppNHp	12.8			Fl
Rab1-AMP∶mGppNHp	9.8			FP
Rab6∶mGDP	9.8	0.048	4.9	FP
Rab6∶mGDP†	3.5	0.018	5.1	FP
Rab6∶mGppNHp	4.6	1.4·10-4	0.03	FP
Rab6-AMP∶mGppNHp	6.7	0.032	4.8	FP
Rab8∶mGDP	39.3	< 10-4*	< 0.01*	Fl
Rab8-AMP∶mGDP	16			FP
Rab8∶mGppNHp	43.6	< 10-4*	< 0.01*	Fl
Rab8-AMP∶mGppNHp	12.8			FP

^*Values are only estimates; FP: fluorescence polarization; Fl: fluorescence.

^†Determined with full-length LidA.