Fig. 3.
Effect of 2-AG on α1β2δ GABAA receptors. (A) Concentration response curve of 2-AG at α1β2δ GABAA receptors. Increasing concentrations of 2-AG (upper bars) were preapplied for 30 s and subsequently coapplied with 1 μM GABA (EC∼8; lower bars) after two control applications of GABA. Concentrations of 2-AG are indicated above the upper bars. Original current traces are shown. Please note that preapplication of 2-AG resulted in small currents by itself. Currents did not saturate completely within the time of application and saturation of potentiation was not reached experimentally. Time of application and concentrations used were both limited by the available 2-AG. (B) Three concentration–response curves of 2-AG were averaged. Mean values ± SD are shown for each point.