Table 2.
Detection of rAAV Vector Sequences by PCR in GC1KO Tissue Samples
| M1* |
M2† |
M3† |
M4‡ |
M5‡ |
M6‡ |
M7‡ |
M8‡ |
M9† |
M10‡ |
M11† |
M12‡ |
|---|---|---|---|---|---|---|---|---|---|---|---|
| AAV8(733)-hGRK1 | AAV8(733)-hGRK1 | AAV5-smCBA | AAV5-smCBA | AAV5-smCBA | AAV5-smCBA | AAV5-smCBA | AAV5-smCBA | AAV5-hGRK1 | AAV5-hGRK1 | No Tx | No Tx |
| N/A | N/A | +160 | +1000 | +970 | +1890 | N/A | +395 | +3140 | +1695 | — | — |
| +632 | +25,885 | — | N/A | N/A | N/A | N/A | N/A | — | N/A | N/A | N/A |
| — | — | — | — | — | — | — | — | — | — | — | — |
| — | — | — | — | — | — | — | — | +252 | — | — | — |
AAV8(733)-hGRK1-mGC1 injections were performed in left eyes; AAV5-mGC1 injections were performed in right eyes. M, animal; Tx, treatment; —, no PCR amplification; +, PCR amplification of vector sequence (replicated value shown is average, copy number per microgram of DN/A); N/A, not available (not determinable because of an unacceptable spike-in).
*
Four months after injection.
†
Seven months after injection.
‡
Ten months after injection.