Skip to main content
. 2011 Sep 13;52(10):7248–7255. doi: 10.1167/iovs.11-7398

Figure 1.

Figure 1.

The MEK inhibitor AZD6244 suppresses the growth of BRAF-mutant and Gα-mutant UM cell lines. (A) The BRAF-mutant OCM3 cells, the GNAQ-mutant OMM1.3 and Mel202 cells, and the GNA11-mutant OMM1 cells were treated with AZD6244 at the indicated concentrations for 96 hours in medium containing 10% FBS. Cell number was quantified with the MTT assay and expressed as percentage of control (DMSO) wells (average ± SD). The BRAF-mutant CM cell lines A375 and M14 served as controls. The BRAF-mutant OCM3 cells were very sensitive to AZD6244, similar to their CM counterparts. The Gα-mutant UM cell lines were less sensitive than the BRAF-mutant cells but still responded with growth arrest. (B) Immunoblot analysis revealed that AZD6244 (1 μM) decreased levels of phosphorylated ERK, decreased levels of cyclin D1, increased levels of p27, and decreased levels of phosphorylated Rb in both BRAF-mutant (OCM3) and Gα-mutant (OMM1.3) UM cell lines.