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. 2011 Nov 3;6(11):e26901. doi: 10.1371/journal.pone.0026901

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Golé et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The diffusion coefficient is decreasing with cell density and experimental time, highlighting the role played by a QSF factors in cell motion. Error bars represent SEM. (A) plotted as a function of the experimental cell density. Cells were recorded during 50 min, 1.5 hours after cells were washed in a fresh HL5 medium. Inset: Average values and corresponding error bars obtained for different cell density ranges; (B) ‘Aging’ assay: as time increases QSF increases and decreases; (C), Evolution of when medium is changed during experiment starting from Highly conditioned Medium (left part, during time [150–300 min], low ), moderate conditioned medium (middle part, during time [350–500 min], intermediate ) and fresh medium (right part, during time [550–700 min], rapid increase in , followed by an exponential decrease); (D) Evolution of with flow . A home-made macrofluidic chamber enables renewing the flow. Applied flow is changed with exponential step (green line, vertical right axis). is first decreasing (corresponding to a state where QSF cell emission is overcoming QSF flow dilution), then increasing (corresponding to a state where QSF flow dilution is overcoming QSF cell emission). When flow is stopped after 700 min, rapidly decreases (corresponding to a rapid increase in QSF concentration).

Inline graphic — The diffusion coefficient is decreasing with cell density and experimental time, highlighting the role played by a QSF factors in cell motion. Error bars represent SEM. (A) plotted as a function of the experimental cell density. Cells were recorded during 50 min, 1.5 hours after cells were washed in a fresh HL5 medium. Inset: Average values and corresponding error bars obtained for different cell density ranges; (B) ‘Aging’ assay: as time increases QSF increases and decreases; (C), Evolution of when medium is changed during experiment starting from Highly conditioned Medium (left part, during time [150–300 min], low ), moderate conditioned medium (middle part, during time [350–500 min], intermediate ) and fresh medium (right part, during time [550–700 min], rapid increase in , followed by an exponential decrease); (D) Evolution of with flow . A home-made macrofluidic chamber enables renewing the flow. Applied flow is changed with exponential step (green line, vertical right axis). is first decreasing (corresponding to a state where QSF cell emission is overcoming QSF flow dilution), then increasing (corresponding to a state where QSF flow dilution is overcoming QSF cell emission). When flow is stopped after 700 min, rapidly decreases (corresponding to a rapid increase in QSF concentration).