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. 2011 Nov 3;6(11):e27246. doi: 10.1371/journal.pone.0027246

Figure 3. Southern blot analysis of PVL phage genomic DNA from strain 68111 and strain ATCC49775.

Figure 3

Phage DNA from MSSA68111 (Lanes designated “6”) and from strain ATCC49775 (Lanes “A”) was digested with either XbaI (A.) or SpeI (B.). In both A and B, the panels on the left are photographs of ethidium bromide-stained gels; the panels on the right are Southern blots hybridized with the lukS-PV probe. 1 kb DNA ladders (Lanes “L”) are shown. The lukS-PV probe hybridized to identical 2 kb fragments in XbaI -digested phage DNA from both strains (A, arrow) whereas SpeI-digestion produced different sized lukS-PV fragments (B.).