Figure 4. DNA sequences of integration site of PVL phage in bacterial genome.
(A.) PCR was performed with primers, as shown in (B.) using bacterial genomic DNA as templates. PCR products were separated by agarose gel electrophoresis and stained with ethidium bromide. (C.) The sequence of PCR product from MSSA68111 was determined and is shown relative to other strains carrying PVL phage. The sequence originating from the phage PVL genomic DNA including lukF-PV gene is printed in upper-case letters, and that derived from the bacterial chromosome, a conserved hypothetical protein, is shown in italic letters. Twenty-nine base pairs of core attR sequence are bolded and underlined. Nucleotide variations are underlined. 1.ФPVLv68111; 2. ΦPVL (strain ATCC49775 [25]); 3. ΦPVL108 (Accession: AB243556); 4. Φ2958 PVL (Accession:AP009363); 5. ΦSLT (Accession: CP000255); 6. ΦSa2 MW (Accession: BA000033).
