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. 2011 Nov 3;6(11):e27287. doi: 10.1371/journal.pone.0027287

Figure 1. Effect of purified bovine NPC2 on cholesterol accumulation in wild type and NPC2−/− fibroblasts.

Figure 1

(A) SDS-PAGE of 5 µg purified NPC2 resolved in a 10–20% gradient gel under nonreducing conditions and stained with Coomassie Brilliant Blue (lane 1). Molecular mass markers are shown on the left (lane M). (B) Upper row human- (Hu) and lower row murine (Mu) fibroblasts cultivated for 48 hours in complete medium (DMEM + 10% FBS). Wild type fibroblasts (left panels), NPC2-/- fibroblasts (middle panels), NPC2-/- fibroblasts supplemented with 600 nM NPC2 (right panels). Cells were fixed with 10% phosphate buffered formalin, pH 7.4 and stained with Filipin III and visualized using fluorescence microscope.