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. 2011 Nov 3;6(11):e27287. doi: 10.1371/journal.pone.0027287

Figure 2. Immune response to NPC2 in 129P2 wild type- and NPC2−/− mice.

Figure 2

NPC2 coated microtiter wells were incubated with serial dilutions of immunized serum as indicated. Bound antibodies were detected by TRIFMA as described in Materials and Methods. An arbitrary concentration of anti-NPC2 antibodies was set to 1000 mU/ml in positive control serum prepared by subcutaneously injections of NPC2 with Freund's complete adjuvant as immune potentiator. Sera from saline treated healthy mice were similarly tested and served as negative controls. (A) Anti-NPC2 antibody concentration in intraperitoneal injected wild type mice, (B) Anti-NPC2 antibody concentration in intravenous injected wild type mice, and (C) Anti-NPC2 antibody concentration in intravenous NPC2 treated NPC2−/− mice. Data represents mean values of triplicate wells.