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. 2011 Nov 3;6(11):e27287. doi: 10.1371/journal.pone.0027287

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Nielsen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Staining of representative cerebellum sections from saline-treated wild type mice (left panels), saline treated NPC2^−/− mice (middle panels), and NPC2 treated NPC2^−/− mice (right panels). Hematoxylin-eosin (H&E) staining (first row), immunohistochemical GFAP-staining for detection of astrocytes (brownish) (second row), and immunohistochemical localisation of antigen F4/80 positive macrophages (brownish) (third row). Data are representative of three separate experiments. n = 3 animals in each experimental group. Scale bars represent 30 µm.