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. 2011 Nov 3;7(11):e1002352. doi: 10.1371/journal.pgen.1002352

Figure 2. Artificial microRNA knockdown of tso1-1 transcripts suppresses tso1-1 phenotype.

Figure 2

(A) A tso1-1 mutant inflorescence showing flower buds with jagged sepals. (B) An inflorescence of tso1-1; amiRTSO1 line #1 showing suppressed floral phenotype. (C) An inflorescence of tso1-1; amiRTSO1 line #7 with an even more suppressed floral phenotype. (D) Side view of a tso1-1; amiRTSO1 line #7 plant showing short and seedless siliques (arrows). (E) A wild type (Ler) inflorescence. (F) A tso1-1/+; amiRTSO1 inflorescence. (G) A +/+; amiRTSO1 inflorescence. (H) Real time RT-PCR analysis of tso1-1 transcript levels in tso1-1; amiRTSO1 (white bars) or tso1-1 (black bars) plants. The TSO1 transcript level in wild type (Ler, grey bars) is also shown. The relative level of tso1-1 transcripts in tso1-1 plants was designated as 1. Standard deviation was calculated based on three technical replicates.