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. 2011 Nov 3;7(11):e1002352. doi: 10.1371/journal.pgen.1002352

Figure 4. Analyses of T-DNA insertion lines in TSO1, SOL1, and SOL2.

Figure 4

(A) Positions of mutations in TSO1, SOL1, and SOL2. Full-length genomic sequences, including promoters and 5′ and 3′ UTRs, are shown. Dark boxes represent exons. Light boxes indicate the UTRs. Grey thin lines represent introns. Black lines represent promoters. Black-filled triangles mark the position of T-DNA insertions or tso1 alleles. tso1-5 (Salk_102956) T-DNA is inserted in the seventh intron very close to the exon-intron boundary. sol1-1 (Salk_007957) is inserted in the SOL1 promoter. sol1-2 (Salk 013686) is inserted in the SOL1 5′UTR. sol2-1 (Sail 78_A12) and sol2-2 (Salk_021952) are both inserted in the first intron of SOL2. tso1-2 missense allele mutates a conserved cysteine in the first CXC domain. tso1-3 is a nonsense mutation in the hinge region between the two CXC domains. tso1-1 mutates a conserved cysteine located in the second CXC domain. (B–D) Real time RT-PCR analysis of SOL2, SOL1, and TSO1. Standard deviations were calculated based on two biological replicates, each with three technical replicates. The wild type level for each gene was assigned as value 1.