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. 2011 Nov 15;138(22):4979–4990. doi: 10.1242/dev.068155

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© 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 6. — Dpy19l1 knockdown does not affect neuronal differentiation. E14.5 mouse embryos were electroporated with control (A-A″ ,E) or Dpy19l1 shRNA (sh1769, B-B″ ,F-H″ ) and analyzed at E16.5 (A-B″ ) or P2 (E-H″ ). (A-B″ ) BrdU incorporation in VZ cells at E16.5. (C) Quantification of BrdU-labeled cells. The percentage of GFP and BrdU double-positive cells in the basal region of the VZ (brackets in A′,B′) is plotted as the mean ± s.e.m. (three embryos each for control and sh1769). (D) Quantification of Cux1-positive cells. The percentage of GFP and Cux1 double-positive cells in the E17.5 CP-IZ is plotted as the mean ± s.e.m. (three embryos each for control and sh1769). (E-H″ ) Double immunostaining of GFP and Cux1 at P2. In control embryos, most GFP-positive cells were immunolabeled by Cux1. In Dpy19l1-downregulated brains, Cux1 was expressed in GFP-positive cells that had stalled abnormally in the IZ and the CP, as well as in neurons that were correctly positioned in the upper cortex. Boxed areas in F are magnified in G-H″ . Scale bars: 20 μm in A-B″ ,G-H″ ; 100 μm in E,F.