Figure 4. The impaired relaxation to ACh induced by visfatin in rat microvessels does not depend on the insulin receptor.

The microvessels were pre-incubated with visfatin (50 ng/mL) alone or combined with either an antibody blocking the activation of the insulin receptor (InsR Ab; 2 µg/mL) and then contracted with 1 µmol/L NA and exposed to increasing concentrations of ACh (100 pmol/L to 3 µmol/L). Results are expressed as mean ± SEM of 20 segments obtained from 4 animals. *P<0.05 vs control curve.