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. 2011 Nov 3;7(11):e1002341. doi: 10.1371/journal.ppat.1002341

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Ito et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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BM-derived DCs (BMDCs) (A) and BMDMs (M) (B) were stimulated with H1N1 (MOI = 10) for 6 hours, then quantitative real-time PCR was performed and the expression levels of Notch ligands were evaluated. BMDC (CD11b⁺CD11c⁺) (C) and BMDM (CD11b⁺F4/80⁺) (D) were stimulated with PolyI:C (10 µg/ml), LPS (1 μg/ml), CpG (1 µM), or H1N1 (MOI = 10) for 24 hours, then flow cytometry was performed as indicated in MATERIALS and METHODS. *P<0.05, **P<0.01 compared with PBS-treated cells. Data shown indicate mean±SEM and are from a representative experiment of 3 independent experiments. Each time point represents at least 4 mice per group. N.D. = No Detection, MFI = Mean Fluorescence Intensity.

Inline graphic — BM-derived DCs (BMDCs) (A) and BMDMs (M) (B) were stimulated with H1N1 (MOI = 10) for 6 hours, then quantitative real-time PCR was performed and the expression levels of Notch ligands were evaluated. BMDC (CD11b⁺CD11c⁺) (C) and BMDM (CD11b⁺F4/80⁺) (D) were stimulated with PolyI:C (10 µg/ml), LPS (1 μg/ml), CpG (1 µM), or H1N1 (MOI = 10) for 24 hours, then flow cytometry was performed as indicated in MATERIALS and METHODS. *P<0.05, **P<0.01 compared with PBS-treated cells. Data shown indicate mean±SEM and are from a representative experiment of 3 independent experiments. Each time point represents at least 4 mice per group. N.D. = No Detection, MFI = Mean Fluorescence Intensity.