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. 2011 Nov 3;7(11):e1002341. doi: 10.1371/journal.ppat.1002341

Figure 2. Dll1 expression expression is dependent upon type-I IFN.

Figure 2

(A) Gene expression of Dll1 on BMDMs from WT, TRIF−/−, MyD88−/−, or IFNαR−/− mice following stimulation with PolyI:C (10 µg/ml), LPS (1 μg/ml), CpG (1μM), H1N1 (MOI = 10), recombinant (r) IFN-α (20 Units), or rIFN-β (20 Units) for 6 hours. *P<0.05, **P<0.01 compared with WT mice. (B) The gene expression level of Dll1 on BMDMs pretreated with anti-mouse IFN-β Ab (2 µg/ml). *P<0.05, **P<0.01 compared with control (cont) Ab. (C) The level of Dll1 on BMDMs (CD11b+F4/80+) following stimulation with H1N1 (MOI = 10) for 24 hours determined by flow cytometry. **P<0.01 compared with PBS-treated BMDMs. (D) Confocal immunofluorescent examination of BMDMs stimulated with H1N1 (MOI = 10) between WT and IFNαR−/− mice. F4/80: green, Dll1: red, DAPI: blue. Original magnification, ×400. Data shown are mean ± SEM and are a representative experiment of 3 independent experiments. Each point represents at least 3 mice per group.