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. 2011 Nov 3;7(11):e1002341. doi: 10.1371/journal.ppat.1002341

Figure 3. RIG-I- and JAK/STAT-dependent Dll1 induction in BMDMs.

Figure 3

(A–C) BMDMs were transfected with RIG-I siRNA or control siRNA. The cells were then incubated with H1N1 for 24 hours (A, B) or for 6 hours (C), and the production of IFN-α (A) and IFN-β (B) was determined by ELISA, and the expression of Dll1 gene was measured by quantitative real-time PCR (C). **P<0.01 compared with control siRNA treated BMDMs. N.D.  =  not detectable (D) BMDMs were stimulated with PolyI:C (10 µg/ml), LPS (1 µg/ml), H1N1 (MOI = 10), or rIFN-β (20 Units) for 6 hours, and then expression of STAT1, phosphorylated (p)STAT1, STAT2, pSTAT2, and Dll1 were measured by western blotting. GAPDH was used as a loading control. (E, F) BMDMs were stimulated with PolyI:C (10 µg/ml), H1N1 (MOI = 10), or rIFN-β (20 Units) for 6 hours, and then Dll1 gene expression was analyzed by quantitative real-time PCR. (E) Dll1 expression on BMDMs between WT and STAT1−/− mice. **P<0.01 compared with WT (129S6) mice. (F) Dll1 expression on BMDMs between DMSO and JAK-I inhibitor treatment (10 µM). **P<0.01 compared with DMSO treatment. Data shown are mean ± SEM and are a representative experiment of 3 independent experiments. Each point represents at least 4 mice per group.