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Semi-quantitative assay of purified PvNod41 proteolytic activity
| Protein substrates | Efficiency of cleavage (n = 5) |
|---|---|
| Casein | 58% (± 2%) |
| Denatured casein | 67% (± 5%) |
| BSA | n.c. |
| Denatured BSA | n.c. |
| Gelatin | n.c. |
n.c. not cleaved.
Proteolytic activity of purified PvNod41 was tested against several model substrates. The assays were performed as described in "Methods". Efficiency of cleavage was compared to that of trypsin.
