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. 2011 Nov 3;7(11):e1002356. doi: 10.1371/journal.ppat.1002356

Figure 2. Direct and dose-dependent role of LLO in L. monocytogenes entry.

Figure 2

(A) LLO-deficient L. monocytogenes (Δhly, strain DPL2161, L.m.) treated with 1 mM nickel (II) chloride coating buffer in the absence (C) or presence of LLO (black bars) or LLO Alexa 488 (grey bar) were used to infect HepG2 cells at 37°C for 30 min (MOI = 20). (B) HepG2 cells were infected with LLO-deficient L. monocytogenes (Δhly, DPL2161) at 37°C for 30 min (MOI = 20), in the absence (C) or in the presence of LLO added exogenously to the cell culture medium (black bars). In (A) and (B) bacterial entry was measured by fluorescence microscopy. The results were expressed relative to bacteria incubated with host cells in the absence of LLO (C) and were the mean ± SEM (n≥3).