Figure 7. Transmission of TgMHu2ME199K prions to wt mice.

a: Picture of a wt C57BL/6 mouse infected with brain homogenates from TgMHu2ME199K/wt mice showing abnormal hind limb posture. Upper panel shows foot prints of the mice as compared to wt mice, demonstrating abnormal pattern of walking. b: Survival curves for wt C57BL/6 mice infected i.p with a TgMHu2ME199K/wt sick mouse brain (group 9.9, close square) or a TgMHu2ME199K/wt asymptomatic mouse brain (group 9.3, open square), as well as i.c (close circles) or i.p (open circles) infected with a TgMHu2ME199K/ko sick mouse brain, or i.p with the brain homogenate of TgMHu₂M mice (close diamond) and i.c with wt brain homogenate (open triangle). c: Brain homogenates of RML infected and wt mice as well as mice (1&2) infected with TgMHu2ME199K/wt brain homogenate (group 9.3) immunoblotted in the presence and absence of PK digestion with α PrP pAb RTC. d: Brain homogenates digested in the presence or absence of PK and immunoblotted with α PrP pAb RTC of the brain samples described in table 3. Upper panel: −PK, lower Panel: +PK overdeveloped. Compare sample 1 in figures c and d to appreciate the overdevelopment factor.