Figure 6. Arabidopsis plants expressing constitutively HaRxLs accumulate less ROS and/or callose in response to flg22.

(A) Leaf discs from four-week-old transgenic plants expressing the indicated HaRxL were sampled and floated in water 14 to 16 h prior to flg22 treatment. Photon emission was measured every 100 milliseconds for 40 minutes. Lines and error bars represent the mean of maximum values of photon counts ±2× SE of 24 independent leaf discs. This experiment was repeated four times with similar results. (B) Leaves of four-week-old transgenic lines were hand inoculated with 100 nM of flg22. Twenty-four hours post-inoculation, leaf discs were sampled and stained with aniline blue for visualization of callose dots. The bars represent mean ±2× SE of callose dots per image photographed (field of 0.22 square centimeters). Callose dots were quantified with ImageJ. Twenty leaf discs were analyzed per transgenic line. This experiment was repeated three times with similar results.