Skip to main content
. 2011 Nov 3;7(11):e1002258. doi: 10.1371/journal.pcbi.1002258

Figure 8. Validation of differential expression of disease associated genes in an unseen disease model.

Figure 8

Results from qPCR experiments measuring differences in gene expression between control cells (WTA, N = 3 independent cultures) and cells expressing the OPMD-associated PABPN1 with expanded repeat (D7E, N = 3 independent cultures). Expression levels were normalized to Desmin to correct for differences in the myogenicity in the different cell cultures. Significant differences (P<0.05, Student's T-test) between measured expression values in D7E and WTA cells are indicated by *, whilst NS stands for no significant difference. PA28α, RPT3, RPN15, RPN11, β2, and β5 expression in IM2 cell lines were selected from the group of genes present in the interspecies disease domain. PA28β (deregulated in human dataset) was selected as its role in assembling the lid subunit of the immunoproteasome is highly similar to PA28α but not part of the interspecies disease domain. β2i is one of the two genes that remained connected to the class node in the interspecies disease domain constructed by naïve Dandelion approach. ACTA1 is a control for myotube formation.