Table 1.
Primers used in RNA expression
| gene | Sequences | Tm (°C) | Product Size(bp) | |
|---|---|---|---|---|
| QPCR | GAPDH | F:5'GGGAAACTGTGGCGTGAT3' R:5'GAGTGGGTGTCGCTGTTGA3' |
59 | 299 |
| FHIT | F:5'GGAGATCAGAGGAGGAAATGG3' R:5'GGGAGTTGGAGTGACCGAG3' |
59 | 233 | |
| PTEN | F:5'ACACGACGGGAAGACAAGTT3' R:5'CTGGTCCTGGTATGAAGAATG3' |
59 | 157 | |
| CHFR | F:5'GCGTAGAAATGCCCAAACC3' R:5'TCCATCCAGCCCGAGTAGC3' |
59 | 171 | |
| SFRP4 | F:5'GGCCTCTTGATGTTGACTGTAA3' R:5'GAGGGATGGGTGATGAGGA3' |
59 | 204 | |
| PAX1 | F:5'GGTAGGAGTAGGGAGCACAGG3' R:5'CAAGTGTTGCGAGTGGAGG3' |
59 | 100 | |
| TSLC1 | F:5'TTATTTCAGGGACTTCAGGC3' R:5'TTCCACCGCAGTGTCTTTC3' |
59 | 223 | |
| CCNA1 | F:5'GCCTGGCAAACTATACTGTGAAC3' R:5'GTGCAGAAGCCTATGACGATTA3' |
59 | 295 | |