Table 1.
Clone name3 | M. smegmatis mc2155b | Gene product/function | Insertion sitec | M. Tuberculosis ortholog(% identity)*d |
---|---|---|---|---|
Ml | MSMEG_4806 | putative acyl-CoA | 201 | NFe |
M2 | MSMEG6781 | hypothetical protein | 681 | NF |
M3 | MSMEG_4727 | Mycocerosic Acid synthase | 2723 | Rv1527(65%) |
M4 | MSMEG_6215 | Manganese containing catalase | 252 | NF |
M5 | MSMEG_5925 | Riesce(2Fe-2S) domain protein | 608 | Rv3526 (65%) |
M6 | MSMEG_3215 | ABC transporter ATP-binding protein | 518 | NF |
M7 | MSMEG_5714 | Short-chain dehydrogenase/reductase SDR | 104 | NF |
M8 | MSMEG_0200 | Hypotetical protein | 779 | NF |
M9 | MSMEG_6105 | Cell division protein | 447 | Rv3610c (86%) |
M10 | MSMEG_0831 | Short-chain dehydrogenase | 159 | NF |
Mil | MSMEG_6611 | Hypotetical protein | 110 | NF |
M12 | MSMEG_0304 | Acyl-CoA synthase | 177 | Rv1427c (77%) |
M13 | MSMEG_0228 | Adenylate and Guanilate cyclase domain protein | 1417 | NF |
SI | MSMEG_3808 | UvrA exinuclease, ABC, A subunit | 866 | Rv1638 (88%) |
S2 | MSMEG_3808 | UvrA exinuclease, ABC, A subunit | 959 | Rv1638 (88%) |
S3 | MSMEG_4293 | Glutammate-ammonia-ligase adenylyltransferase | 883 | Rv2221c(73%) |
aSelected mutants moderately (M) or severely affected (S)
bGenes are indicated as annotated by TIGR/GeneBank database (Accession number C000480.1)
cRelative to the first base of the putative coding sequence
dCut off identity was set at 60%
e Not found