Table 1.

Genes disrupted in M and S mutants identified ( LM)-PCR

Clone name3	M. smegmatis mc2155b	Gene product/function	Insertion sitec	M. Tuberculosis ortholog(% identity)*d
Ml	MSMEG_4806	putative acyl-CoA	201	NFe
M2	MSMEG6781	hypothetical protein	681	NF
M3	MSMEG_4727	Mycocerosic Acid synthase	2723	Rv1527(65%)
M4	MSMEG_6215	Manganese containing catalase	252	NF
M5	MSMEG_5925	Riesce(2Fe-2S) domain protein	608	Rv3526 (65%)
M6	MSMEG_3215	ABC transporter ATP-binding protein	518	NF
M7	MSMEG_5714	Short-chain dehydrogenase/reductase SDR	104	NF
M8	MSMEG_0200	Hypotetical protein	779	NF
M9	MSMEG_6105	Cell division protein	447	Rv3610c (86%)
M10	MSMEG_0831	Short-chain dehydrogenase	159	NF
Mil	MSMEG_6611	Hypotetical protein	110	NF
M12	MSMEG_0304	Acyl-CoA synthase	177	Rv1427c (77%)
M13	MSMEG_0228	Adenylate and Guanilate cyclase domain protein	1417	NF
SI	MSMEG_3808	UvrA exinuclease, ABC, A subunit	866	Rv1638 (88%)
S2	MSMEG_3808	UvrA exinuclease, ABC, A subunit	959	Rv1638 (88%)
S3	MSMEG_4293	Glutammate-ammonia-ligase adenylyltransferase	883	Rv2221c(73%)

^aSelected mutants moderately (M) or severely affected (S)

^bGenes are indicated as annotated by TIGR/GeneBank database (Accession number C000480.1)

^cRelative to the first base of the putative coding sequence

^dCut off identity was set at 60%

^e Not found