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Expression of NOX2 and p22phox was increased during I/R. WT mice underwent I/R or sham surgery, and retinas were collected after 3, 6, 12, 24, and 48 hours. (A) The mRNA levels of different NOX subtypes, p47phox, and p22phox subunits were quantified at 6 hours after retinal ischemic injury by q-PCR and were normalized to WT sham surgeries (n = 4; **P < 0.01 vs. WT sham). (B) NOX2 protein levels were determined at different time points during I/R injury (n = 8; *P < 0.05 vs. 0 hour).
