Fig. 11.
Ultraviolet-visible absorption spectra of the mixture containing ferrous ion, 2-PCA and EDTA. The condition of the ultraviolet-visible absorption measurements were as described in Materials and Methods.
A, 1.4 mM Fe2+ + 7 mM 2-PCA; B, 1.4 mM Fe3+ + 1.4 mM NADPH + 7 mM 2-PCA; C, 1.4 mM Fe3+ + 1.4 mM NADPH + 1.7 mM EDTA + 7 mM 2-PCA.