Figure 1.
Generation and morphology of conditional Tbr2 ablation in the corneal epithelium of Tbr2ceΔ/ceΔ mice. (A) To prepare mice lacking TGF-β receptor type II (Tbr2) in the corneal epithelium, Tbr2 floxed mice were bred to Krt12-Cre mice. Arrows: primers used for genotyping and detection of gene excision. (B) Genotyping and excision PCR of corneal epithelium. Tbr2 gene of Krt12Cre/Cre/Tbr2f/f mouse corneal epithelium was excised completely. NC, no DNA control; M, molecular size markers. (C) Tbr2 Western blot analysis. (D) Tbr2 immunofluorescence. Red: Tbr2 expression; green: nuclear counterstain. (E–J) Corneal morphology by hematoxylin-eosin staining reveals a relatively normal-looking corneal epithelium with dividing cells located in the basal epithelium after loss of Tbr2. (E–G) Low magnification. (H–J) High magnification. Krt12 expression did not change in the absence of Tbr2 (K–M). Red: Krt12.