Figure 3.

Activation of ERK1/2 by FGF-2 stimulation through PI 3-kinase. The serum-starved hCECs were pretreated with PI 3-kinase inhibitor or MEK1/2 inhibitor for 2 hours and then maintained in DMEM with FGF-2 for 8 hours. At the end of treatment, cells were lysed and then immunoblotted with the designated antibody. ERK1/2 works as a downstream regulator to the PI 3-kinase/Akt pathways triggered by FGF-2 stimulation. Total Akt and ERK1/2 were used to control protein concentration on immunoblot analysis. The results represent data obtained in three independent experiments.