Skip to main content
. 2011 Oct 17;52(11):8216–8223. doi: 10.1167/iovs.11-8213

Figure 5.

Figure 5.

Inhibitory effect of siRNA to KIS on phosphorylation of p27 at the Ser10 site, but not at the Thr187 site. Human CECs were transfected with KIS siRNA. After transfection, cells were maintained with DMEM or DMEM with FGF-2 for an additional 24 hours. Total protein extracts were prepared and immunoblotted with designated antibodies. Actin was used to control protein concentration on immunoblot analysis. The results represent data obtained in three independent experiments. C, negative control cells which were transfected with negative control siRNA (Silencer Select Negative Control; Ambion).