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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Nutr Biochem. 2011 Jul 20;23(6):635–639. doi: 10.1016/j.jnutbio.2011.03.007

FIGURE 2.

FIGURE 2

The 3’UTR of HCS mRNA contains a miR-153 binding site. (A) Sequence alignment of miR-153 (top row) and its putative binding site in the 3’UTR of HCS mRNA in humans and other species (rows 2-6). The box denotes the most highly conserved region. Luciferase activity (B) and mRNA abundance (C) was quantified in HEK-293 cells that were stably transformed with Ecd-mir153-CMV-Luc-HCS3UTR and treated with PonA for 48 h; controls were cultured in the absence of PonA. Values are means ± SD, n= 3. Means without a common letter differ, P < 0.05.