Figure 3.

IGFBP-3NB regulates vascular permeability in vivo. (A) Quantification of fluorescent leakage after either VEGF (40 ng/μL) alone, VEGF followed by IGFBP-3NB (100 nanograms/microliter), IGFBP-3NB alone, or vehicle for VEGF (saline) injection. VEGF injection increased permeability compared with vehicle (saline)-injected eyes. When IGFBP-3NB was intravitreally injected post VEGF treatment, retinal vascular permeability was decreased compared with the VEGF-injected only control. (B-E) Representative retinal flat-mounts from animals described in Fig. 2A. (B) Saline, (C) VEGF, (D) VEGF followed by IGFBP-3NB, and (E) IGFBP-3NB. Arrows indicate representative areas of vascular leakages.