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. 2011 Oct 21;52(11):8278–8286. doi: 10.1167/iovs.11-8167

Figure 5.

Figure 5.

IGFBP-3NB maintains tight junction integrity by maintaining claudin-5 expression in mice injected with VEGF. (A–O) Immunohistochemical analysis of retinal flat-mounts for claudin-5 expression at magnification ×60. The red stain is claudin-5 and the green stain is FITC-conjugated albumin used to visualize the retinal blood vessels. (A–C) Saline-injected vehicle control retinas showing expression of claudin-5 levels in the retinal vessels. (D–F) IGFBP-3NB-injected retinas showing expression of claudin-5 levels in the retinal vessels. (G–I) VEGF-injected retina showing a significant loss of claudin-5 expression in the retinal vessels. (J–L) VEGF-injected retina followed by saline injection 6 hours later. There is significant loss of claudin-5 expression in the retinal vessels. (M–O) VEGF-injected retina followed by IGFBP-3NB injection 6 hours later. There is preservation of claudin-5 expression levels in the retinal vessels when IGFBP-3NB is intravitreally administered to VEGF-injured retinas.