Table II.

Effect of malonyl-CoA upon [1¹⁴C]3-OH C20:0-CoA dehydration and elongation

Condition	(E)-2,3 C20:1-CoA Synthesis	Saturated Acyl-CoAs
		Synthesis	Label distribution
			C20:0	C22:0	C24:0
	nmol mg−1 h−1		%
Control	57.0  (100)	NDa	ND	ND	ND
+NADH	61.4  (88.3)	8.1  (11.7)	100	ND	ND
+NADPH	9.2  (10.2)	81.4  (89.8)	73	22	5

The assays were carried out in the presence of 9 μm malonyl-CoA in the same conditions as in Table I. Fatty acids were analyzed by radio-GLC and reversed TLC as reported in Methods.

^aND, Not determined.