Table 2.
Elevated Proteins in TGFβ2-Treated Human TM Cells
| SwissProt Accession | Protein | Subcellular Source | Sample Frequency (Total = 4) | Mean Protein Ratio | SEM | P |
|---|---|---|---|---|---|---|
| ECM Remodeling | ||||||
| P09486 | SPARC† | AC | 3 | 6.70* | 0.14 | 0.006 |
| P29279 | Connective tissue growth factor | A | 4 | 2.94* | 0.18 | 0.009 |
| P02461 | Collagen α1 (III) | A | 4 | 2.89* | 0.13 | 0.004 |
| O00469 | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2† | C | 4 | 2.58* | 0.09 | 0.002 |
| P07996 | Thrombospondin-1† | A | 3 | 2.41* | 0.15 | 0.030 |
| Q16270 | Insulin-like growth factor-binding protein 7 | A | 3 | 2.40* | 0.17 | 0.036 |
| O15460 | Prolyl 4-hydroxylase subunit α2† | C | 4 | 2.11* | 0.22 | 0.042 |
| Q96CG8 | Collagen triple helix repeat-containing protein 1 | A | 3 | 2.04* | 0.14 | 0.036 |
| P13674 | Prolyl 4-hydroxylase subunit α1† | C | 4 | 1.53 | 0.14 | 0.057 |
| P55145 | Mesencephalic astrocyte-derived neurotrophic factor | A | 4 | 1.45 | 0.08 | 0.020 |
| Cytoskeletal Interactions | ||||||
| P24844 | Myosin regulatory light chain 9† | B | 4 | 2.12* | 0.11 | 0.006 |
| Q05682 | Caldesmon | B | 4 | 2.06* | 0.21 | 0.042 |
| Q8WX93 | Palladin | B | 4 | 1.70 | 0.06 | 0.003 |
| Q9HBL0 | Tensin-1 | B | 3 | 1.67 | 0.07 | 0.017 |
| P37802 | Transgelin-2 | B | 4 | 1.56 | 0.08 | 0.012 |
| Q9Y2B0 | Protein canopy homolog 2 | C | 3 | 1.45 | 0.03 | 0.008 |
| Protein Folding and Chaperone Processes | ||||||
| P02511 | α-Crystallin B | B | 4 | 3.37* | 0.16 | 0.004 |
| Q8NBS9 | Thioredoxin domain-containing protein 5 | C | 4 | 1.66 | 0.06 | 0.003 |
| Q9Y4L1 | Hypoxia upregulated protein 1 | C | 4 | 1.49 | 0.09 | 0.020 |
| Q96AY3 | FK506-binding protein 10† | C | 4 | 1.48 | 0.05 | 0.005 |
| P11021 | 78 kDa glucose-regulated protein | C | 4 | 1.43 | 0.06 | 0.008 |
| Q15084 | Protein disulfide-isomerase A6 | C | 4 | 1.41 | 0.10 | 0.041 |
| Regulatory Processes | ||||||
| P50479 | PDZ and LIM domain protein 4† | B | 4 | 1.68 | 0.14 | 0.036 |
| Q14192 | Four and a half LIM domain protein 2† | BD | 4 | 1.67 | 0.09 | 0.012 |
| Q9P2E9 | Ribosome-binding protein 1 | C | 4 | 1.65 | 0.07 | 0.006 |
| O00264 | Membrane-associated progesterone receptor component 1 | C | 4 | 1.53 | 0.14 | 0.059 |
| P62851 | 40S ribosomal protein S25 | B | 4 | 1.50 | 0.12 | 0.045 |
| O94925 | Glutaminase, kidney isoform | E | 3 | 1.43 | 0.07 | 0.037 |
| P24534 | Elongation factor 1-β | B | 4 | 1.42 | 0.06 | 0.009 |
| O15173 | Membrane-associated progesterone receptor component 2 | C | 4 | 1.39 | 0.10 | 0.052 |
Adjusted mean protein ratios (TGFβ2-treated/control), standard error of the mean (SEM). P values, and subcellular source for abundant proteins (from Supplementary Table S5 [http://www.iovs.org/lookup/suppl/doi:10.1167/iovs.11-8218/-/DCSupplemental]). Abundant proteins exhibited mean ratios at least 1 SD above the mean and P < 0.06 (Fig. 2). All proteins were quantified in ≥3 TM cell samples (median ratio = 1.00; mean = 1.01; SD = 0.31; n = 450).
*
Ratios at least 2 SD above the mean. Protein subcellular source from the Swiss Protein database: A, secreted; B, cytoplasmic; C, membrane; D, nuclear; E, mitochondrial.
†
Calcium or metal ion-binding proteins.