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. Author manuscript; available in PMC: 2012 Sep 8.
Published in final edited form as: Oncogene. 2011 Aug 1;31(10):1207–1216. doi: 10.1038/onc.2011.325

Figure 4.

Figure 4

Ionizing radiation selectively enriched the 1.5 kb MnSOD transcript. A. Exponential cultures of MB-231 cells were irradiated with 2-8 Gy and harvested at 48 h post-irradiation for quantitative RT-PCR measurements of MnSOD mRNA levels. Asterisks represent statistical significance relative to MnSOD mRNA levels in unirradiated controls; n=3, p<0.05. B. Control and 8 Gy irradiated MB-231 cells were harvested at indicated times and MnSOD mRNA levels analyzed using a quantitative RT-PCR assay. Fold change calculated relative to un-irradiated control at the time of irradiation (0 h). The quantitative RT-PCR assay was repeated to measure the abundance of the 4.2 kb and 1.5 kb MnSOD transcripts, panels C and D, respectively. Asterisks represent statistical significance relative to 0 h control; ¥ represents statistical significance relative to 24 and 48 h samples; n=3, p<0.05.