Figure 4. Cathepsin E enhances the suppression of T_conv cells by TRAIL.

293T cells were transfected either with Ctse and Tnfsf10 alone or together. The cells were irradiated with 3000 rads 48h post transfection and seeded at a density of 7000 cells per well in a 96 well flat bottom plate. Freshly isolated C57BL/6 T_conv cells were added to the seeded plate at 8×10⁴ per well and stimulated with anti-CD3 and anti-CD28 coated beads for 72 hours. Proliferation of responder cells was determined by [³H]-thymidine incorporation. T_conv cell proliferation was calculated by subtracting the basal [3H]-thymidine incorporation of irradiated 293T plus T cells without anti-CD3 and anti-CD28 stimulation. Data represent the average of three independent experiments. p-value, * : <0.05.