Figure 5. TRAIL dependence and modulation in Ebi3^−/− Il10^−/−T_regs.

Wild type or knock out T_reg purified by FACS were activated in presence of anti-CD3 and anti-CD28 coated latex beads with IL2 for 16 and 24h. (A) Cells were collected and surface TRAIL expression detected by flow cytometry using an anti-mouse TRAIL antibody. Data are representative of 3 independent experiments. (B) Mean fluorescence intensity (MFI) of surface TRAIL expression following activation from 3–4 independent experiments were plotted. Students t Test; p-value ** = <0.05. (C) Wild type or knock out T_reg were cultured in the insert of a Transwell™ culture plate in the presence of wild type T_conv cells. zVAD or DMSO control was added to the Transwell™ assay. Freshly purified wild type responder T_conv cells were activated in the bottom chamber of the plate. Proliferation of responder cells was determined by [³H]-thymidine incorporation. Data represent the mean ± SEM of 2 independent experiments. p-value * = 0.07.